Markers related to undifferentiated ES cell contamination were not found in reverse transcriptase–polymerase chain reaction analysis of the Percoll-enriched cardiomyocytes. Histological, immunohistochemical, and transmission electron microscopic studies further indicate that the ECTs both structurally and functionally resemble neonatal native cardiac muscle. After in vitro stretching for 7 days, the ECT can beat synchronously and respond to physical and pharmaceutical stimulation. The enriched cardiomyocytes were mixed with liquid type I collagen supplemented with Matrigel to construct engineered cardiac tissue (ECT). The ES cell–derived cardiomyocytes were then enriched by Percoll gradient centrifugation. The embryoid bodies were then induced to differentiate into cardiomyocytes in a medium supplemented with 1% ascorbic acid. Methods and Results- ES-D3 cells were cultured in a slow-turning lateral vessel for mass production of embryoid bodies. However, despite recent progress in in vitro differentiation and in vivo transplantation methodologies of ES cells, to date, no one has succeeded in using ES cells in tissue engineering for generation of somatic tissues in vitro for potential transplantation therapy. Customer Service and Ordering Informationīackground- Embryonic stem (ES) cells can terminally differentiate into all types of somatic cells and are considered a promising source of seed cells for tissue engineering.Stroke: Vascular and Interventional Neurology.Journal of the American Heart Association (JAHA).Circ: Cardiovascular Quality & Outcomes.Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB).

Embryoid body blood island